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Fig. 5. Regulation of en2 expression and β-catenin stability by PP2A:B56ϵ in the neural ectoderm at stage 14. (A) RT-PCR showing that en2 is reduced by PP2A:B56ϵ morpholino at stage 14. (B-E) Whole-mount in situ hybridization showing pax2.1 (B,C) and XHR1 (D,E) are not reduced by PP2A:B56ϵ morpholino injection at stage 14. (B,D) Control embryos; (C,E) embryos injected with PP2A:B56ϵ morpholino unilaterally. (F) Western blot showing injected β-catenin was less stable in the neural ectoderm of PP2A:B56ϵ knockdown embryos. EFGP was used as a control for both injection and loading. |
