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Figure 2. Activation of FGFR2 Signaling Increases the Size of the Neural Tube and Changes Retinal Fates to Ventral Neural Fates(A and B) Neural tube is thicker on the cFGFR2-injected side (*). os, expanded optic stalk.(C) White lines indicate extent of sox3 expression on control (right) and cFGFR2-injected (*) sides of neural plate.(D) D1.1.1 clone (green) in stage 14 forebrain region of control. Arrow denotes midline.(E) D1.1.1 clone (green) in the same region of cFGFR2-injected embryo.(F) Expanded neural tube on cFGFR2-injected side (*). Brown stain indicates HNK-1 expression.(G) Expression of cFGFR2 in D1.1.1 clone (red) expands pax2 expression on the injected side (*) of neural plate.(H) Expression of cFGFR2 in D1.1.1 expands forebrain pax2 expression (white bracket) on the injected side (inj) of tail bud embryos. un, uninjected.(I) Number of dopamine cells in the hypothalamus of cFGFR2-injected embryos. *, p < 0.001.(J) The lengths of dorsal (black line) and ventral (Nkx2.4+; white line) forebrain domains in cFGFR2-injected and wild-type (wt) embryos.
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