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Fig. 1. Effect of XSmo on the proximodistal patterning of the visual system. Lateral view of the head region of embryos (st. 36) injected with mRNAs
encoding XSmo (1.5 ng; A, B, D, E), XSmo-M2 (1.5 ng; F), or Shh (500 pg; G) into one of two blastomeres. (C) Transverse vibratome section of an
XSmo-injected embryo at the level of the optic vesicles. (D-G) XPax-2 expression (purple) in manipulated embryos detected by whole-mount in situ
hybridization. In (E) and (G), injected sides (is) were identified by beta-galactosidase staining (light blue). (H) In situ hybridization revealing ectopic XPtc1
expression in embryos injected unilaterally (one of two blastomeres) with mRNAs coding for XSmo (H, 1.5 ng, 86%, n 28 embryos). is, injected side;
nis, noninjected side; ov, optic vesicle; rpe, retinal pigmented epithelium. |
