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Fig. 2. XSmo induces ectopic otic vesicles expressing XPax-2. Embryos injected with XSmo mRNA (1.5 ng) into one of two blastomeres were fixed at
embryonic stage 36 and stained for XPax-2 expression (purple) by whole-mount in situ hybridization. Lateral (A�C, E) and dorsolateral (D) views of injected
embryos showing ectopic vesicular structures expressing XPax-2 in -galactosidase-positive (light blue) manipulated sides (arrowheads, 12%, n 50). (F)
Horizontal vibratome section of the embryo shown in (D) at the level of the otic vesicles revealing the vesicular morphology of the ectopic otic vesicle. The
single otic vesicle of the noninjected side appeared in a section plane localized more ventrally (not shown).
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