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Figure 3. Partial depletion of Dzip1 sensitizes embryos to the Smo agonist purmorphamine. (A) Whole mount in situ hybridization of ptc1 (top), pax6 (middle) and pax2 (bottom) in vehicle- treated (ethanol) controls and embryos treated with either a low dose of purmorphamine (2.5 - 5.0 μm), a high dose of purmorphamine (10 μm), injected with a low concentration of DMO (3 ng) or receiving a combination of a low dose of purmorphamine and an injection with a low concentration of DMO. In the middle panels, the distance between the floor plate and the ventral border of the pax6 expression domain was highlighted. (B-D) Summary of ptc1 expression phenotypes (B), pax6 expression phenotypes (C) or pax2 expression phenotypes (D) for vehicle treated (ethanol) controls and the different groups of embryos subjected to purmorphamine, DMO or both. |
