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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
not
|
|
tropicalis
|
NF stage 28
|
forebrain
,
telencephalon
,
branchial arch
,
chordoneural hinge
,
pineal gland
,
[+]
|
hoxb3
|
|
tropicalis
|
NF stage 28
|
presomitic mesoderm
,
rostral presomitic mesoderm
|
sox3
|
|
tropicalis
|
NF stage 28
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
spinal cord
,
[+]
|
dlx5
|
|
tropicalis
|
NF stage 28
|
neural crest
,
branchial arch
,
cranial neural crest
|
ap2a1
|
|
tropicalis
|
NF stage 14
|
neural crest
,
cranial neural crest
|
myod1
|
|
tropicalis
|
NF stage 14
|
mesoderm
,
presomitic mesoderm
,
paraxial mesoderm
|
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S3 Fig. Patterning and Apoptosis in nol11 morphants.
A) Somite, CNC and neural development appear intact in nol11 morphants at stage 14 as assayed by myoD, ap2 and pax2 expression. Expression of xnot appears reduced at stage 28. B) Reduced dlx5 expression and BA hypoplasia on treated side of a stage 28 embryo. Neural patterning is normal at this stage as assayed by expression of pax2, hoxb3 and sox3. C) Whole mount TUNEL staining of treated and untreated sides of a stage 28 embryo. Note the increased staining in the craniofacial regions of the morphant side.
doi:10.1371/journal.pgen.1005018.s003 |
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Display additional annotations [+]
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S1 Fig. nol11 in vertebrate development.
A) Whole mount in situ hybridization of digoxigenin labelled Nol11 probe in E9.5 and E10.5 mouse embryos (E10.5 sample shown has been hemisected). BA, branchial arch; FNP, frontonasal prominence; Ht, heart; mdBA1, mandibular BA1; mxBA1, maxillary BA1; Op, optic placode; Ot, otic placode. B) Gross morphology of stage 45 nol11 morphants. Gut morphology is abnormal in morphants, while organ situs appears largely normal relative to wild type controls. Heart (red), gall bladder (green) and gut (yellow) are pseudocoloured in lower right panels. C) Left sided expression nkx2.5 is reduced or absent in the splenic anlage of a subset of nol11 morphants (compare red arrowheads). D) Example of the normal sided pitx2 expression present in nol11 knocked down embryos. E) Kidney development appears largely intact in nol11 MO treated side compared to control side. F) Quantification of number of embryos displaying the described phenotypes.
doi:10.1371/journal.pgen.1005018.s001 |