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Fig. 7. RNA-Seq identification of Prdm12-regulated genes. (A) Experimental setup for the RNA-Seq analysis. (B) Selected ontological classification of genes regulated by Prdm12. (C) Examples of identified differentially expressed genes. (D) Venn diagram showing the overlap of differentially regulated genes between Prdm12, Prdm12-EnR and Prdm12-VP16. (E) Validation of some of the identified genes as prdm12 target genes by RT-PCR in RA-treated neuralized animal caps. (F) pax2 and gad1 on sections of embryos injected with either mouse Prdm12 mRNA (200 pg/blastomere) or with a Prdm12 MO. IS, injected side. (G) RT-qPCR analysis of pax2 and gad1 in EnR-Prdm12- and VP16-Prdm12-overexpressing RA-treated neuralized caps. |
