| |
Figure 7. Characterization of the rescued-Mo, failed-rescued and altered (-MTS)-induced pronephros development. A,B: GFP observation illustrates the correct targeting of mesodermal derivatives up to the end of tadpole stage. Arrows indicates gfp-positive tubules. C–F: pax8 whole-mount ISH at early tail bud stage in embryos injected with MmHspa9 alone or in combination with Mo17. G–J: pax2 whole-mount ISH at early tadpole stage in control and Mo17+MmHspa9 injected embryos. K,L: smp30 whole-mount ISH in early tadpole embryos injected with Mo17+MmHspa9. A stronger signal is present on all rescued embryos (arrows). M,N: pax2 whole-mount ISH in early tadpole embryos injected with Mo17+MmHspa9(-MTS). Arrows point to a lack of pax2 positive pronephros and arrowheads to a simplified proximal tubule. O,P: GFP signals were missing in pronephros area of MmHspa9(-MTS) injected tadpoles. Q,R: pax8 expression is severely reduced in MmHspa9(-MTS) injected embryos at stage 21. S–V: Reduced or absent pax2 signals are observed in MmHspa9(-MTS) injected tadpoles. W: Histograms represent the frequency of normal, weakly and severely modified pronephros in all groups (Mo, n = 78; Mm, n = 38; Mo+Mm, n = 50; Mm (-MTS), n = 40; Mo+Mm(-MTS), n = 41).Download figure to PowerPoint |
