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pax2xenopus anterior dorsal lateral plate region 

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Experiment details for pax2

Li DH et al. (2005) Assay

The role of XTRAP-gamma in Xenopus pronephros development.

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Gene Clone Species Stages Anatomy
pax2.L laevis NF stage 29 and 30 to NF stage 31 anterior dorsal lateral plate region

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  Fig. 5. Whole-mount in situ hybridization shows the effect on expression of pronephros marker genes XTRAP-γ MO injection. Embryos were injected with XTRAP- γ MO (40 ng) or 5-mis-XTRAP-γ MO (40 ng) and synthetic of β-galactosidase mRNA (250 ng), then fixed at stage 30/31 (A-P) and stage 22/23 (Q-T). Expression of Lim-1 was increased in the side of embryos injected with XTRAP-γ MO (A), compared the non-injected side (B). Expression of Pax-2 and Wnt-4 was inhibited by injection of XTRAP- γ MO (E,I), compared with noninjected side (F,J). For those embryos fixed at stage 22/23, expression of Pax-2 (Q) and Wnt-4 (S) in embryos fixed at stage 22/23 was not affected by injection of XTRAP- γ MO or the control 5-mis-XTRAP- γ MO (R,T). Expression of Pax-8 was not affected by XTRAP-γ MO (M,N). The expression of Lim-1 (C,D), Pax-2 (G,H), Wnt-4 (K,L) and Pax-8 (O,P) was unchanged in embryos injected with 5-mis- XTRAP-γ MO.
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