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Fig. 8. The engrailed repressor domain of lbx1 is necessary for its function. (A-F) mRNA from a mutant lbx1 construct that lacks the engrailed homology domain was injected into one cell at the two-cell stage. The injected sides are on the right. Phenotypes opposite of wild-type lbx1 overexpression were observed. At stage 24, a transverse section reveals an increase in myoD staining on the injected side (A, arrow), while a transverse section through a stage 31 tadpole exhibits a decrease in Pax3 staining (B, arrow). Transverse sections through stage 29 tadpoles (C-F) were stained with the pH3 antibody alone (C,E) or with 12/101 (D,F). No increase in the number of mitotic nuclei is observed on the injected sides (C,E), and 12/101 staining (D,F) indicates a slightly shorter, yet wider, differentiated myotome (arrows). |
