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Fig. 5. NC induction by Wnt signalling is Gbx2 dependent. Embryos were injected in animal blastomeres at the eight-cell stage as indicated. The expression of Snail2, Pax3 and Msx1 was analysed at stage 12. (A-C) β-catenin-GR (1 ng) and induced at stage 10 with DEX. Between 75 and 86% of NC expansion; n=153 embryos. (D-F)β -catenin-GR (1 ng), induced at stage 10 with DEX and 16 ng of Gbx2 MO. NC expansion was reduced to less than 2%; n=174. (G-I) 1 ng of Dsh dominant-negative DD1. Between 78 and 82% of inhibition of NC genes; n=124. (J-L) Dsh dominant-negative DD1 (1 ng) and 1 ng of Gbx2 mRNA. NC inhibition was reduced to less than 2%; n=120. |
