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Fig. 9. Co-injection of myoD mRNA with lbx1 mRNA eliminates overproliferation and pax3 upregulation. (A- O) Embryos were injected into one cell at the two-cell stage with, lbx1 (A-C), myoD (D-F), myf5 (G-I), lbx1 + myoD (J-L) and lbx1 + myf5 (M-O). Injected embryos were grown until stage 29, sectioned in the transverse plane, and analyzed with anti-phospho-histone H3 antibody (A,D,G,J,M), 12/101 (B,E,H,K,N) or pax3 expression (C,F,I,L,O). The injected side of each tadpole is on the right, marked by Beta- galactosidase (red). Anti-phospho-histone H3 and 12/101 staining were performed on the same sections, while pax3 staining was carried out on different tadpoles. Injection of lbx1 causes an increase in proliferation (A), decrease in differentiated muscle (B) and increase in pax3 (C). Injection of myoD causes no change in proliferation (D), an increase in differentiated muscle (E) and a decrease in pax3 expression (F). Injection of myf5 causes no significant change in cell proliferation (G), a slight increase in differentiated muscle (H) and no change in pax3 expression (I). Injection of lbx1 + myoD (J-L) produces a phenotype consistent with injection of myoD alone, while injection of lbx1 + myf5 (M-O) causes a phenotype like that of lbx1 alone. |
