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Fig. 3. Neural crest markers in embryos with modulated Dvl and Ror2 function. (A-H)Xenopus embryos were injected with the indicated Dvl (Dsh construct) RNAs (1 ng each) and processed for in situ hybridization as described in Fig. 1. Schematic representations of the Dvl mutant constructs are shown at the top. Dsh-delta-N activates Sox8 (A) and FoxD3 (B) but not Sox2 (C). Dsh-DEP+ slightly expands Sox2 (D) but inhibits Sox8 (E), FoxD3 (F), Pax3 (G) and AP2 (H). (I-L)The effects of Ror2 interference on NC marker expression. Ror2-delta-C and Ror2MO, but not wild-type Ror2, inhibit FoxD3 (I,K,L). MyoD is not affected by Ror2-delta-C (J). Arrows point to altered marker expression. Dorso-anterior view is shown. |
