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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
zic1
|
|
laevis
|
NF stage 18
|
cranial neural crest
,
neural plate border
|
sox3
|
|
laevis
|
NF stage 20
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
anterior neural fold
,
anterior placodal area
|
foxd3
|
|
laevis
|
NF stage 18
|
cranial neural crest
|
msx1
|
|
laevis
|
NF stage 18
|
neural plate border
,
anterior placodal area
|
tubb2b
|
|
laevis
|
NF stage 18
|
Rohon-Beard neuron
,
neuron
,
anterior placodal area
|
sox9
|
|
laevis
|
NF stage 18
|
neural crest
,
trunk neural crest
,
cranial neural crest
,
anterior placodal area
|
|
|
Fig. 4. Effects of Dlx3 or GATA2 gain or loss of function on neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. For Msx1, additional examples of embryos are shown in the insets in C and C′. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gata2
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
|
eya1
|
|
laevis
|
NF stage 18
|
anterior placodal area
,
posterior placodal area
|
six1
|
|
laevis
|
NF stage 18
|
anterior placodal area
,
posterior placodal area
|
zic1
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
posterior placodal area
|
sox3
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
,
neural plate border
,
anterior placodal area
|
foxd3
|
|
laevis
|
NF stage 18
|
cranial neural crest
|
msx1
|
|
laevis
|
NF stage 18
|
neural plate
,
presumptive midbrain-hindbrain boundary
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
,
[+]
|
dlx5
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
foxi2
|
|
laevis
|
NF stage 18
|
anterior placodal area
|
sox9
|
|
laevis
|
NF stage 18
|
epibranchial placode
,
cranial neural crest
,
posterior placodal area
|
dlx3
|
|
laevis
|
NF stage 18
|
non-neural ectoderm
,
pre-chordal neural plate border
,
chordal neural plate border
,
neural plate border
|
krt12.4
|
|
laevis
|
NF stage 18
|
epidermis
,
non-neural ectoderm
|
|
|
Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details. |
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Display additional annotations [+]
|
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Fig. S1. Expression of Pax genes is not induced in non-neural ectoderm. (A-C) After grafting neural plates (NP) from stage 13 pigmented donors into the belly ectoderm (B) of stage 13 albino hosts, Pax gene expression was analyzed in tailbud stage embryos. Grafts (outlined) are shown in high magnification views. Expression of Pax6, Pax3 and Pax2 is confined to grafts. (D-H) After grafting belly ectoderm (B) from stage 13 pigmented donors into the lateral neural plate border (NB) of stage 13 albino hosts, expression of Pax genes was analyzed in tailbud stage embryos. None of the Pax genes analyzed is induced in the graft (E is a transverse section through the embryo in D, showing absence of Pax6 in graft; insets in D,H show control sides). |