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pax3xenopus   

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Experiment details for pax3

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Differential distribution of competence for panplacodal and neural crest induction to non-neural and neural ectoderm.

Gene Clone Species Stages Anatomy
pax3.S laevis NF stage 18 pre-chordal neural plate border , chordal neural plate border , neural plate border , anterior placodal area

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  Fig. 4. Effects of Dlx3 or GATA2 gain or loss of function on neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. For Msx1, additional examples of embryos are shown in the insets in C and C′.

Gene Clone Species Stages Anatomy
pax3.S laevis NF stage 18 chordal neural plate , profundal placode , pre-chordal neural plate border , chordal neural plate border , neural plate border

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  Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details.

Gene Clone Species Stages Anatomy
pax3.S laevis NF stage 22 ectoderm , pre-chordal neural plate , chordal neural plate , neural plate , profundal placode , [+]

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  Fig. S1. Expression of Pax genes is not induced in non-neural ectoderm. (A-C) After grafting neural plates (NP) from stage 13 pigmented donors into the belly ectoderm (B) of stage 13 albino hosts, Pax gene expression was analyzed in tailbud stage embryos. Grafts (outlined) are shown in high magnification views. Expression of Pax6, Pax3 and Pax2 is confined to grafts. (D-H) After grafting belly ectoderm (B) from stage 13 pigmented donors into the lateral neural plate border (NB) of stage 13 albino hosts, expression of Pax genes was analyzed in tailbud stage embryos. None of the Pax genes analyzed is induced in the graft (E is a transverse section through the embryo in D, showing absence of Pax6 in graft; insets in D,H show control sides).