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Fig. 3.
xREST-DBD decreases the expression of other neuronal genes but does not perturb neural induction.a–c, ISH for N-tubulin; the injected side is marked by an asterisk; b, both lateral views;c, anterior view of the same embryo, where thearrowheads signal the label of the primary spinal neurons (sn) and cranial ganglia (cg).d, xREST-DBD represses SCG10 expression.Double arrowheads signal the expected normal position of N-tubulin or SCG10 expression. The spotted labeling in the injected side (a) and thediffuse hue in c result from β-galactosidase activity staining. e–h, xREST-DBD does not affect neural induction and early differentiation. Expression patterns of Sox2 (e), Slug (f), Hox11L12 (g), and Pax-3 (h). e, h, Anterior views; f, g, dorsal views. |
