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Figure 8. xCdc4δFbox inhibits neural crest development. xCdc4δFbox or xCdc4 mRNA (1 ng) was injected into one cell of two-cell-stage embryos. GFP mRNA (1 ng) was injected as a control, and β-gal mRNA was injected as a lineage tracer (light blue or red unilateral staining). (A-U) Whole mount ISH was performed for Snail2 (A-C), Snail (D-F), c-Myc (G-I), Pax3 (J-L), Opl (M-O) and Msx1 (P-R), and the early neuronal marker Sox2 (S-U). Representative embryos are shown (anterior view, dorsal up, injected side right). |