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Fig. 2. Pcdh7 knockdown disrupts expression of neural crest specification markers, but not neural crest induction or neural platemarkers. Embryoswere injected with Pcdh7MO, Pcdh7δE
orCMO, together with LacZmRNA, fixed at stage 15–17, stained for β-galactosidase, and subjected to in situ hybridization for neuralmarkers. Neither Pcdh7MO nor Pcdh7δE has an effect
on expression of the inductionmarkers Pax3 (A, B), Zic1 (C,D) or c-myc (E, F), nor on the neural plate markers Sox15 and Sox2 (G, H). In contrast, Pcdh7MO- or Pcdh7δE-injectedembryos
exhibit reduced expression of the specification markers Sox9 (I, J), Snail2 (K, L) and Twist (M, N). Control (CMO)-injected embryos show no change in specificationmarkers Snail2 (O) or
Twist (P). Anterior is towards the top of the photos and the injected side of the embryos is to the right. |
