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Fig. 7. rx1 is not required after stage 12 for other retinal stem or neural identity marker genes but is necessary for the onset of differentiation markers. (A) pax6 expression domain in the optic vesicle (arrow) is smaller (cf. Fig. 5D). (B) At stage 38, the pax6 expression domain is normal, and no repression surrounds β-Gal-labeled nuclei (arrowheads). (C) six3 expression domain in the optic vesicle (arrow) is smaller (cf. Fig. 5F). (D) At stage 38, there is no repression of six3 surrounding the β-Gal-labeled nuclei (arrowheads). (E) notch1 expression domain in the optic vesicle is smaller (arrow). However, cells with β-Gal labeled nuclei (inset, arrowheads) express notch1. (F) At stage 38, notch1 expression is similar to controls and no repression surrounds β-Gal labeled nuclei (arrowheads). (G) sox2 expression domain in optic vesicle (arrow) is smaller (cf. Fig. 5J). (H) At stage 38, there is no repression of sox2 expression surrounding the β-Gal labeled nuclei (arrowheads). (I) ngn1 expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5L). (J) At stage 38, ngn1 expression continues to be repressed surrounding β-Gal labeled nuclei (arrowheads). (K) p27 expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5N). (L) At stage 38, p27 expression is repressed surrounding β-Gal labeled nuclei (arrowheads) in some embryos. (M) neuroD expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5P). Inset: cells with β-Gal-labeled nuclei (arrowheads) do not express neuroD. (N) At stage 38, neuroD expression continues to be repressed in β-Gal-labeled cells (arrowheads). (O) Xath5 expression in the optic cup (arrow) is greatly reduced (cf. Fig. 5R). Inset: cells with β-Gal-labeled nuclei (arrowheads) do not express Xath5. (P) At stage 38, Xath5 continues to be repressed in β-Gal-labeled cells (arrowheads). Data for stage 16 activation are similar albeit at higher frequencies for differentiation genes (see Table 1). Dashed lines indicate the outline of the retinas in the sectioned material. For whole mounts, bars = 100 μm; for sections bars = 20 μm. |
