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pax6xenopus   

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Experiment details for pax6

Neural induction in Xenopus requires inhibition of Wnt-beta-catenin signaling.

Neural induction in Xenopus requires inhibition of Wnt-beta-catenin signaling.

Gene Clone Species Stages Anatomy
pax6.L laevis NF stage 18 diencephalon , eye primordium , neural fold

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  Fig. 4. δXTcf3-mediated expansion of the neural plate can be uncoupled from effects on A patterning. (A) Embryos injected into one cell at the eight-cell stage with mRNA encoding δXTcf3 show expanded expression of Sox3 along the entire A axis (i, ii). However, sibling embryos show little or no expansion of Otx expression (iii, iv); note the lineage tracer, β-gal (light blue stain) along the entire A axis and highlighted by red arrow. (B) At doses of δXTcf3 RNA that expand Sox3 (i), the mesoderm marker Muscle Actin (ii) appears relatively unaffected on the injected side (arrow). A neural markers show normal expression on the injected side (arrow) relative to contralateral control. Markers examined are (iii) Krox20; (iv) En-2; (v) Pax6; (vi) BF-1: (vii) Xanf (viii) Otx with "p." denoting probe used.