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Figure 2 Expression of Pax-6 in the developing brain. In all panels except (E,F), anterior is
to the right. In all panels except (A), (C), and (H), dorsal is up; in (A,C,H), dorsal is facing
the viewer. (A) The earliest apparent staining pattern of Pax-6 at the neural plate stage (stage
14); arrow indicates the medial stripe. (B,C) A lateral (B) and dorsal (C) view of a stage 23
embryo. The arrowhead indicates the optic vesicle in (B), while the arrows indicate the two
rhombomere-specific stripes in the hindbrain in (C). The midbrain staining is visible in (C)
medial to the dotted white lines. (D) A lateral low-power view of a stage 28 embryo. The
arrowhead indicates the gap in Pax-6 expression in the mesencephalon (optic tectum). The
arrow points out the staining in the presumptive oculomotor nucleus. The dotted white line
serves to distinguish the eye from the telencephalon/diencephalon. The developing eye also shows Pax-6 expression. (E) A transverse section of a stage 28 embryo at the level of the
diencephalon: the arrow indicates staining in the dorsal diencephalon. (F) A transverse section
of a stage 28 embryo through the spinal cord. The arrow indicates Pax-6 staining in the
ventricular zone. N notochord. (G) A lateral view of a stage 42 embryo. The arrowhead
shows a decrease of Pax-6 expression in the spinal cord, while the arrow shows a decrease of
expression in the outermost layer of the retina. (H,I) A dorsal (H) and lateral (I) view of a
stage 42 brain dissected free of surrounding tissue. The arrowhead in (H) points to staining in
the olfactory bulb, while the arrows point to the rhombomere-specific staining. Abbreviations
in (I): My meyelencephalon, Me mesencephalon, Di diencephalon, Te telencephalon.
Scale bar is 200 mm in all except (E,F) (100 mm). |
