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FIGURE 6. Analysis of progenitor cells at embryonic stages (BLBP, Lhx2, Sox2, Pax6, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at embryonic stages showing the codistribution of the progenitor markers BLBP (A,C), Lhx2 (B,C,E–G), Sox2 (D), and Pax6 (E,F′); and the combination of Lhx2 with the mitotic marker PH3 (G,H). In each panel the developmental stage and the color code for the used markers are indicated. (F,F′) Are confocal images, and the higher magnification shown in (F’) corresponds to the framed area indicated in (F). The BLBP (A) and the Lhx2 (B) labeling detected at stage 37/38 showed double labeled cells exclusively in the ventricle (C). The combination of Lhx2 and Sox2 also showed double labeled cells in the ventricle, but the cells detected for both markers in the mantle were never double labeled (D). In the case of Pax6, Lhx2/Pax6 double labeled cells were observed in the ventricular zone (E,F′), but never away from this region, where both cell populations were intermingled (F′). The labeling of Lhx2 in combination with PH3 showed that only the ventricular cells expressing Lhx2 were mitotic cells (G,H). Scale bars = 50 μm. See list for abbreviations. |
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FIGURE 8. Analysis of progenitor cells at larval stages (Lhx2, Pax6, BLBP). Micrographs of transverse sections through the pallium of Xenopus laevis at the larval stage 54 showing the distribution of the progenitor markers Lhx2 (A–C,F,G), Pax6 (B,C) and BLBP (D–G). In each panel the color code for the used markers is indicated. The higher magnifications (B,E,F) are from the panels indicated in each case (white boxes in A,D). At this stage in the ventricular zone Lhx2 is expressed by virtually all cells (A,F), and those cells co-expressed Pax6 (B,C) and BLBP (G). Lhx2 expressing cells away from the ventricle, like in the medial pallium, were also labeled for Pax6 (B,C), but never expressed BLBP (G). Scale bars = 100 μm (A,D), 50 μm (B,C,E–G). See list for abbreviations. |
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FIGURE 10. Analysis of progenitor cells at larval stages (Tbr2, BLBP, Pax6, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at the larval stage 54 showing the distribution of the markers Tbr2 (A,C,D′,E′,F,K), BLBP (B,C,D,E) and Pax6 (G–J,L), and the combination of Tbr2 with the mitotic marker PH3 (M–P). In each panel the color code for the used markers is indicated. The higher magnifications (D,E) correspond to the framed areas, as indicated (white boxes in A,B). Tbr2 expressing cells were detected close to the ventricle and away from it (A,D′,E′,F,K) but only those cells in the ventricular zone expressed BLBP (D,D′,E,E′) or Pax6 (F–L). The combination of Tbr2 and PH3 showed that there are not Tbr2 mitotic cells away from the ventricle (M–P). Scale bars = 200 μm (A,B,F,G), 100 μm (C,H,J–M), 50 μm (D,E,I,N,O,P). See list for abbreviations. |
