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Figure 4. Validation and GO analysis of highly significant targets. (A) The validation of five highly significant array targets (blue) is plotted against normalized RT-qPCR derived expression profiles (red). Note that lines connecting each time point are meant to serve as a visual aide. (B) Shows expression patterns of RT-qPCR validated targets using whole-mounts in situ hybridization for leptin, xmenf, xcyp26a, sox9 and pdgfa at 0 h, 6 h, 12 h, 24 h, 36 h, 48 h, and 72 hours post-amputation. (C) Heat map of all highly significant and dynamic targets produced a group of 1441 targets, grouped into 12 clusters by their similar expression changes through the array time points. (D) Gene ontology (GO) analysis of the twelve clusters in (C) showed four clusters over-represented with at least three GO terms accompanied by a p-value under 1e-3 and false discovery rate (FDR) under 0.5. Error bars represent standard error of mean (SEM). |
