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Fig. 4. No induction of Pitx2c in dnah9 and dnah5 morphants. Injection into the marginal region of left and right dorsal blastomeres (A) specifically targets the floor plate (B; external view) and GRP (B’; ventral view of dorsal explant). (C–E) Laterality defects in morphants. Wildtype left-asymmetric expression of Pitx2c in Co-MO-injected specimen (C) and absence of lateral plate expression in dnah9-MO-injected tadpole (D). (E) Quantification of results. Note that bilateral and right-asymmetric expression patterns were very rarely encountered, while absence of Nodal cascade induction was found in up to 75% of cases. Please note that all three MOs resulted in similar effects as well as dose-dependency of dnah9-SB-MO. an, animal; d, dorsal; l, left; r, right; v, ventral; veg, vegetal. |
