| |
Fig. 3. Expression of Pitx2c but not Pitx2b during development of left
right asymmetries in the frog Xenopus. Embryos were analyzed by wholemount
in situ hybridization for the expression of Pitx2 using probes speci
for isoform c (A, I) and isoform b (E, M). Lines indicate the
approximate levels of transverse sections. (A) At stage 30 both isoforms
were co-expressed in the brain (B,F). Pitx2c mRNA was found in the left
myocardium (C) and in the l-LPM (D). No speci staining was detectable
in the heart and LPM with a probe speci for Pitx2b (G,H). (I) Coexpression
of isoforms in the brain presists at stage 38 (J,N). Speci
expression of Pitx2c was found on the left side of the looping stomach
(st, K) and intestine (i, L), while a Pitx2b probe did not reveal speci
signals. |
