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Figure 7. Expression of Pitx2 and Tbx1 in the embryo head. A: Pitx2 is detected in the cement gland and stomodeum-pituitary anlagen (bracket) at stage 16/17. It can also be detected in the prechordal mesoderm and in the most anterior part of the head paraxial mesoderm (arrows). At stage 20, the staining is observed in cells associated to the eye, in a region corresponding to the anlagen of extraocular muscles at stage 28–30 (arrowheads). B: At stage 15/16, Tbx1 expression is localized at the border of neurectoderm as two bilateral stripes. On slide section, at stage 15/16, the staining (arrows) appears to be strong at the border of ectoderm and mesoderm but equally present in mesoderm cells. The staining of mesodermal cells is stronger at the next stages. Dash lines define the level of slide sections. Numbers refer to the developmental stages. Anterior views for stages 15/16 and 20, lateral views for stage 22, 24, 28, and 30. Download figure to PowerPoint. |
