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Fig. 1. The Xnr1-dependent transcriptional complex is a potential target of Notch signaling. (A) The experimental strategy. (B) One pg Xnr1 or 50 pg GR-FoxH1-VP16 RNA were injected into a right ventral blastomere of 4-cell-stage embryos with or without 2 ng GR-NICD or 1 ng GR-ESR1 RNA. DEX was added into the culture medium at stage 22. The expression of Pitx2 at stage 25 was tested by whole mount in situ hybridization. (C) The quantitative assessment of the injections in (B). At least three independent experiments were performed. “n” indicates the number of injected embryos. R: Injection into the right side of embryo. |
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Fig. 2. ESR1 directly blocked the expression of Pitx2 gene in the left LPM. (A) The experimental strategy. (B) One ng GR-NICD or 1 ng GR-ESR1 RNA was injected into a left ventral blastomere of 4-cell-stage embryos. DEX was added into the culture medium at stage 22 and CHX was added 30 min before the addition of DEX. (C) The quantitative assessment of the injections in (B). At least three independent experiments were performed. “n” indicates the number of injected embryos. L: Injection into the left side of embryo. |
