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Fig. 8. Rfx2 is required for GRP cilia assembly and the left–right asymmetry. (A–B) Pitx2c expression at stage 26, lateral view. In wildtype embryos, Pitx2c is expressed in the left LPM (A, A′, C). In TTC25 morphants, only 33% of embryos show normal left LPM expression (C), and Pitx2c is often expressed in the right LPM (B′, C). (D–E) Confocal images of the posterior GRP (node) stained with acetylated α-tubulin to visualize cilia and injected with membrane-RFP to outline cells. (F) GRP cilia were measured in confocal images as in D, E for 5 embryos each. Horizontal lines indicate the mean, vertical lines SEM, pb0.0001 Mann–Whitney test. |
