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Fig. 5. Expression of xPKD1 in animal caps treated with activin A and retinoic acid. Presumptive ectoderm (animal caps) were removed from stage 9 blastula
embryos and incubated with activin A (10 ng/mL) and retinoic acid (10 4 M) until sibling embryos reached stage 41. (A) Whole-mount immunostaining
revealed that the treated animal caps contain 3G8-positive tubular structures (pronephric tubules) (B) xPKD1 expression in animal caps incubated in saline
solution without activin A or retinoic acid (lane 1) or in animal caps treated with both activin A and retinoic acid (lane 2). (C). ODC expression was analyzed as
a loading control. |