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pkd1xenopus ovary 

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Experiment details for pkd1

Cloning and expression of the amphibian homologue of the human PKD1 gene.

Cloning and expression of the amphibian homologue of the human PKD1 gene.

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Gene Clone Species Stages Anatomy
pkd1.L laevis NF stage 66 ovary

  Fig. 3. xPKD1 expression in adult tissues. A. RT-PCR experiments were conducted using xPKD1 specific primers (see Materials and methods; the amplicon size is approximately 400 bp) and RNA extracted from ovaries (lane 1), heart (lane 2), kidney (lane 3), liver (lane 4), intestine (lane 5) and lung (lane 6). B. Control RT-PCR experiments were conducted using ODCspecific primers and the same RNAs samples as in A. C. Northern blot analysis of the xPKD1 transcript in RNAs extracted from ovaries (1) or adult kidney (2). The blots were then probed with the 714 bp external PKD1 RT-PCR product. The exposure time was 48 h. The 14 kb xPKD1 transcript is indicated.
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