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Gene/CloneSpeciesStageAnatomy ItemExperimenter
pnhdxenopus dorso-lateral marginal zone 

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Experiment details for pnhd

Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

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Gene Clone Species Stages Anatomy
pnhd.L laevis NF stage 11.5 to NF stage 12 dorso-lateral marginal zone

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  Fig. 4. In situ hybridization shows that the expression patterns of many characterized and candidate Wnt/β-catenin direct target genes resemble wnt8 expression. Genes are grouped based on functional role. (A) Expression of wnt8 at different developmental stages. (B) Expression of a few characterized direct target genes of Wnt/β-catenin at different developmental stages. (C) Expression of selected candidate direct target genes. Stages 11.5-12 and 12.5-13 are blastopore view with dorsal up. Neurula stage is dorsal view with anterior up.

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Gene Clone Species Stages Anatomy
pnhd.L laevis NF stage 11.5 dorso-lateral marginal zone

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  Fig. 5. Expression of Wnt/β-catenin target genes is reduced by dkk-injection. (A, C, E) Normalized counts from RNAseq data comparing uninjected control (UC) and dkk-injected embryos for selected known (A) and candidate (C, E) target genes at stage 11.5 (mid-gastrula). (B, D, F) In situ hybridization showing expression pattern of known (B) and candidate (D, F) target genes. Embryos were injected in the right (asterisk) 2 blastomeres at the 4-cell stage with a total of 100 pg dkk. Blue box indicates that embryo was cleared in either benzyl benzoate:benzoic acid (BB:BA) or benzyl benzoate alone (BB; to reduce transparency and visualize the archenteron). Blastopore view with dorsal up, except pnhd is dorsal view with anterior up.