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Figure S4. Control tissues and genes abutting the anterior hemangioblast are
unaffected in Fli1 morphants.
40 ng Fli1 MO was injected into the DMZ at the 4/8 cell stage and embryos were grown
to stage 17. Rohon Beard neuron expression of Runx1 (A,B) and somite expression of
Lmo2 (C,D) was unchanged in Fli1 morphants. MyoD, Nkx2.5, Blimp1 and Bmp4
expression abuts the anterior hemangioblast but in no case does expression of these genes
increase in the hemangioblast territory (asterisks) in Fli1 morphants (E-L), indicating that
in the morphants re-specification is not the reason for lost hemangioblast gene
expression.
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