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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
prdm1	xenopus

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Experiment details for prdm1

New methods for computational decomposition of whole-mount in situ images enable effective curation of a large, highly redun...

New methods for computational decomposition of whole-mount in situ images enable effective curation of a large, highly redundant collection of Xenopus images.

Gene	Clone	Species	Stages	Anatomy
prdm1.L		laevis	NF stage 1 to NF stage 40	chordal neural plate , paraxial mesoderm , cranial placode , pre-chordal neural plate border , chordal neural plate border

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Fig 2. Graphical depiction of image analysis workflow for selected images with different shape embryos and a range of different background colours and textures. Images show that embryo detection and stain colour analysis is effective independently of a wide range of variation in image background and embryo characteristics. All images were analysed without changing initial parameters. Note image (d) where even the human eye struggles to distinguish the upper border of the embryo from the background. https://doi.org/10.1371/journal.pcbi.1006077.g002

Gene	Clone	Species	Stages	Anatomy
prdm1.L		laevis	NF stage 10 to NF stage 12	ectoderm , animal hemisphere , non-involuting marginal zone
prdm1.L		laevis	NF stage 13 to NF stage 20	neural crest , non-neural ectoderm , preplacodal ectoderm , neural plate border , anterior neural ridge
prdm1.L		laevis	NF stage 22 to NF stage 40	otic vesicle , presomitic mesoderm , somite , branchial arch , eye , [+] tail tip , tail fin , dorsal fin , pharyngeal region

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Fig 5. Developmental progression of expression for selected genes prdm1, ank1, and hoxb3 during Xenopus embryo development. This is the result of applying our suite of image analysis tools to (in this case) the 334 original images for these three genes, and reducing them to a representative set of 65 images, including multiple views of expression patterns from the early spherical stages (pre-Stage 22). https://doi.org/10.1371/journal.pcbi.1006077.g005