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Fig. 6. Immunocytochemical localization of peripherin in regenerating
axons. Transverse sections through the brains of frogs sacrificed
28 days (A–D) and 21 days (E,F) after optic nerve crush were immunostained
with antibodies to a C-terminal peptide derived from Xenopus
peripherin (diluted 1:1000: A,C,E) and to nonphosphorylated
NF-M (S8 after phosphatase treatment, 1:1000: B,D; RMO270, 1:100:
F). Dorsal side is up and the left side is at the left. A: At the optic
chiasm, peripherin-containing fibers run longitudinally in the left,
crushed optic nerve (arrow). In contrast, in the right, uncrushed optic
nerve, peripherin is found only in clusters of glia cells (g). B: On a
neighboring section, regenerating fibers growing outside the degenerating
core (asterisk) stain strongly for NF-M. The degenerating core
was not visible in A because of the level of sectioning. C: A more
posterior section of the same animal as in A and B shows peripherin containing
fibers in the contralateral optic tract of the diencephalon
(arrow). D: A neighboring section to that in C shows the distribution
of NF-M for comparison. E: In the operated optic nerve close to the
chiasm, peripherin is present in the youngest regenerating axons
(arrows), which are located close to the glia limitans, but is absent in
the oldest regenerating axons, which are found close to the degenerating
core (asterisk). F: On a section adjacent to that shown in E,
NF-M is present in older regenerating axons, but is absent from young
regenerating axons close to the glial limitans (dots). g, glia; oc, optic
chiasm; ot, optic tract; p, pial surface; v, ventricle. Scale bars 300
m in D (applies also to A–C) and 100 m in F (applies also to E). |
