| |
Fig. 9. Proliferating cell nuclear antigen (PCNA) immunoreactivity
in a stage 51 tadpole (A,B) and a juvenile frog (D). A illustrates
PCNA immunoreactivity in cells of the central field (cf) of the spinal
cord, whereas, for comparison, C shows a neighboring section that
was labeled by using in situ hybridization for XIF3 mRNA, which was
found in the central field (cf) as well as in ventral motoneurons (m).
After metamorphosis, PCNA immunoreactivity greatly diminished
(D). B shows PCNA immunoreactivity in the retinal ciliary margin (c)
of a stage 51 tadpole (which can be compared to a neighboring section
labeled by in situ hybridization for XIF3 mRNA in Fig. 5A,C). The
tadpole was stained with anti-PCNA at a dilution of 1:5,000, whereas
juvenile frog spinal cord was stained with antibody at a dilution of
1:1,000. p, Pigmented retinal epithelium; i, inner nuclear layer; r,
retinal ganglion cell layer. Scale bars 5 50 mm in B, 150 mm in C (also
applies to A), 300 mm in D. |
