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sall1xenopus   

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Experiment details for sall1

Young JJ et al. (2014) Assay

Spalt-like 4 promotes posterior neural fates via repression of pou5f3 family members in Xenopus.

Gene Clone Species Stages Anatomy
sall1.L image:6862463 laevis NF stage 10 ectoderm , mesoderm , dorsal marginal zone
sall1.L image:6862463 laevis NF stage 10.5 dorsal marginal zone , involuted dorsal mesoderm , upper blastopore lip
sall1.L image:6862463 laevis NF stage 12 mesoderm , circumblastoporal collar , presumptive axial mesoderm
sall1.L image:6862463 laevis NF stage 15 mesoderm , circumblastoporal collar , axial mesoderm , posterior
sall1.L image:6862463 laevis NF stage 18 mesoderm , notochord , posterior

  Figure S4: sall1 is activated by canonical Wnt signaling and expressed during early embryogenesis. (A) qPCR on 5 whole embryos or 15 to 25 animal caps treated according to the conditions indicated on the X-axis. The Y-axis shows expression relative to odc. Error bars: 1 SEM. (B-E) Whole-mount in situ hybridizations of sall1 in Xenopus laevis embryos. (B) Whole mount stage 10 embryo stained for sall1, dorso-vegetal view with the dorsal lip of the blastopore oriented towards the top. (B’) Sagittal section of stage 10.5 embryo stained for sall1 expression, animal pole is to the top and dorsal is to the right. (C-D) Dorsal views of indicated neurula stage embryos, anterior is oriented towards the top. (C’-C’’) Transverse sections of stage 12 embryos stained for sall1, (C’) anterior and (C’’) posterior. (D’-D’’) Transverse sections of stage 15 embryos stained for sall1, (D’) anterior and (D’’) posterior. (E’-E’’) Transverse sections of stage 18 embryos stained for sall1, (E’) anterior and (E’’) posterior. (B’, C’-E’’) 50 μM sections, (C’-E’’) dorsal oriented towards the top. No: notochord, S: somite, PSM: presomitic mesoderm.