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Figure 5. Expression of XlSALL4 during Xenopus hindlimb development and in response to wounding. A,B: Longitudinal hindlimb sections of whole-mount in situ hybridization (WISH) limbs through the anterior–posterior plane of a stage 47/48 limb and a stage 50 limb. Note the absence of XlSALL4 expression in the epidermis and in the distal subepidermal zone (labeled in A with thin arrows). Also note the asymmetry of XlSALL4 mesenchymal expression along the anterior–posterior axis. Magnifications are variable to fit different size limbs into constant boxes. C: WISH of a stage 55 hindlimb wounded on the anterior side at the base of the autopod (thick arrow) 3 days after wounding, showing no expression at the wound site, while also showing the typical stage 55 interdigital XlSALL4 expression pattern. Posterior is up. |
