|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sema3d
|
|
laevis
|
NF stage 16
to
NF stage 19
|
neural crest
,
rhombomere
,
optic field
|
sema3d
|
|
laevis
|
NF stage 23
to
NF stage 27
|
neural crest
,
eye
,
cranial neural crest
,
rhombomere
,
anterior branchial crest
,
[+]
|
sema3d
|
|
laevis
|
NF stage 28
to
NF stage 32
|
neural crest
,
eye
,
cranial neural crest
,
rhombomere
,
anterior branchial crest
,
[+]
|
sema3fl
|
|
laevis
|
NF stage 14
to
NF stage 15
|
notochord
|
sema3fl
|
|
laevis
|
NF stage 16
to
NF stage 19
|
notochord
,
presumptive midbrain-hindbrain boundary
,
preplacodal ectoderm
|
sema3fl
|
|
laevis
|
NF stage 23
to
NF stage 27
|
midbrain-hindbrain boundary
,
notochord
,
branchial arch
,
rhombomere
|
sema3fl
|
|
laevis
|
NF stage 28
to
NF stage 32
|
midbrain-hindbrain boundary
,
notochord
,
branchial arch
,
rhombomere
|
sema3a
|
|
laevis
|
NF stage 14
to
NF stage 15
|
prechordal plate
,
paraxial mesoderm
,
presumptive midbrain-hindbrain boundary
|
sema3a
|
|
laevis
|
NF stage 16
to
NF stage 19
|
prechordal plate
,
paraxial mesoderm
,
presumptive midbrain-hindbrain boundary
|
sema3a
|
|
laevis
|
NF stage 23
to
NF stage 27
|
midbrain-hindbrain boundary
,
head mesoderm
,
presomitic mesoderm
,
somite
,
splanchnic layer of lateral plate mesoderm
|
sema3a
|
|
laevis
|
NF stage 28
to
NF stage 32
|
midbrain-hindbrain boundary
,
head mesoderm
,
presomitic mesoderm
,
somite
,
splanchnic layer of lateral plate mesoderm
|
|
|
Figure 2. Expression of class-3 semaphorins in Xenopus laevis. Gastrula, neurula, and tadpole stage Xenopus laevis embryos were analyzed by whole mount in situ hybridization. Rows represent the expression pattern of the semaphorin indicated at the left side. Columns show different stages of Xenopus laevis development. Gastrula stage 11-12 embryos are shown with the dorsal side up. Neurula stage 14-15 and the left panel of neurula stage 16-19 embryos are shown from the dorsal side. The right panel of stage 16-19 neurula embryos presents an anterior view of the embryo shown at left. ba, branchial arches; e, eye; h, heart; m, somitogenic mesoderm; mh, midbrain hindbrain boundary; nt, neural tube; no, notochord; s, somites; sm, splanchnic mesoderm. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
twist1
|
|
laevis
|
NF stage 28
|
mandibular crest
,
hyoid crest
,
neural crest
,
cranial neural crest
,
anterior branchial crest
,
[+]
|
sema3d
|
|
laevis
|
NF stage 29 and 30
|
otic vesicle
,
midbrain-hindbrain boundary
,
neural crest
,
posterior neural tube
,
cranial placode
,
[+]
|
egr2
|
|
laevis
|
NF stage 28
|
cranial neural crest
,
rhombomere
,
rhombomere R3
,
rhombomere R5
,
posterior branchial crest
|
sema4c
|
|
laevis
|
NF stage 28
|
cement gland
,
neural crest
,
otic placode
,
cranial neural crest
,
anterior branchial crest
,
[+]
|
sema4g
|
|
laevis
|
NF stage 32
|
eye
,
otic placode
|
sema4b
|
|
laevis
|
NF stage 25
|
cement gland
,
neural crest
,
otic placode
,
pronephric mesenchyme
|
sema3fl
|
|
laevis
|
NF stage 28
|
midbrain-hindbrain boundary
,
cranial placode
,
cranial neural crest
,
rhombomere
,
rhombomere R3
,
[+]
|
sema5b
|
|
laevis
|
NF stage 32
|
brain
,
midbrain
,
hindbrain
,
neural crest
,
eye
,
[+]
|
sema3a
|
|
laevis
|
NF stage 25
|
diencephalon
,
midbrain-hindbrain boundary
,
mandibular crest
,
neural crest
,
somite
,
[+]
|
sema3a
|
|
laevis
|
NF stage 29 and 30
|
diencephalon
,
anterior dorsal lateral plate region
|
sema3a
|
|
laevis
|
NF stage 32
|
diencephalon
,
midbrain-hindbrain boundary
,
mandibular crest
,
somite
,
lateral plate mesoderm
,
[+]
|
sema6d
|
|
laevis
|
NF stage 29 and 30
|
otic placode
,
cranial neural crest
,
neural tube
|
sema6a
|
|
laevis
|
NF stage 28
|
otic vesicle
,
midbrain-hindbrain boundary
,
optic vesicle
,
lateral plate mesoderm
,
anterior dorsal lateral plate region
|
sema4a
|
|
laevis
|
NF stage 29 and 30
|
neural crest
,
cranial placode
,
periocular region
|
sema6b
|
|
laevis
|
NF stage 32
|
otic vesicle
,
spinal cord
,
neural crest
,
cardiac neural crest
|
|
|
Figure 7. Semaphorins are expressed in close proximity to migrating cranial neural crest cells. In situ hybridization of tadpole-stage embryos; enlargements of the head are shown. Red arrows indicate migrating neural crest cells, white arrows indicate neurogenic placodes. A: Sema3A expression. Staining in the midbrain hindbrain boundary was confirmed by double in situ hybridization with engrailed (data not shown). B: Sema3C expression. C: Sema3D expression. D: Sema3F expression. Double in situ hybridization using Krox20 or engrailed antisense probes was used to confirm staining in the rhombomeres or the midbrain hindbrain boundary, respectively (data not shown and Q). E: Sema4A expression. F: Sema4B expression. G: Sema4C expression. H: Sema4G expression. I: Sema5B expression. K: Sema6A expression. L: Sema6B expression. M: Sema6D expression. N: Sema3A expression at early tadpole stage. O: Double in situ hybridization using a Sema3A (blue) and a twist (red) antisense probe at tadpole stage. Sema3A expression is localized posterior to the migrating twist-expressing neural crest cells (black arrow). P: For comparison twist expression at tadpole stage is shown. Q: Double in situ hybridization showing Sema3F (blue) expression adjacent to the anterior portion of the migrating Krox20-expressing (red) neural crest cells (black arrow). cg, cement gland; e, eye; mh, midbrain hindbrain boundary; op, otic placode; r3, rhombomere 3; r5, rhombomere 5. |