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Fig. 8. B56ε is required for the expression of endogenous Hh target genes. (A) Whole mount in situ hybridization showing the expression of shh (upper panels) and ptc-1 (lower panels) was reduced by εmor (3.5 ng) injection at stage 14/15. The reduced shh and ptc-1 expression was partially rescued in embryos injected with εmor and ε-c (100 pg). (B) RT-PCR showing εmor (3.5 ng) injection reduced ptc-1, foxA2, and shh expression from late gastrula stage. ODC was the loading control. (C) The expression of ptc-1, ptc-2, foxA2, and shh in tadpole stage control embryos (left column) and embryos injected with 3.5 ng of εmor (right column). Embryos were bilaterally injected at the 8-cell stage. |
