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Figure 5.
Essential Role of the Chordin-Sizzled System in DV Pattern Scaling after Bisection
(A-C) External phenotypes at the tailbud stage in bisection experiments (bisection was done at stage 8). Lateral, dorsal, and ventral views are shown in (A), (B), and (C), respectively.
(D) Transcript levels of regional markers in the same number of control (blue), dorsal-half (red), and ventral-half (green) embryos (stage 12) shown by qPCR (three experiments). Bars represent SD.
(E) Transcript levels of chordin (blue), noggin (red), and sizzled (green) in the same number of control, dorsal-half, and ventral-half embryos at stage 9 (three experiments). Bars represent SD.
(F-M) Effect of Sizzled depletion on DV marker patterns in bisection conditions. (F) through (I) show uninjected controls. (J) through (M) show sizzled-MO-injected embryos. (F), (H), (J), and (L) show whole embryos. (G), (I), (K), and (M) show bisected dorsal halves. (F), (G), (J), and (K) show dorsal views. (H), (I), (L), and (M) show lateral views. (I) Balanced shh and sizzled expression in 100%, n = 11. (L) shows dramatic sizzled expansion in 100%, n = 20. (M) shows disproportionate reduction in sizzled expression in 100%, n = 12.
See also Figure S5. |
