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FIG. 3. Localization of Xshh mRNAs by whole mount in situ
hybridization. (A) Uninjected embryos. (B) Cross-sectioning
through the trunk of A. (C) Embryos injected with 10 pg Xnr-1
mRNA. Xshh mRNAs were expressed only in the primary axis. (D)
Cross-sectioning through the trunk of C. Neural tissue consisting
of the structure of the tube was induced (arrowhead). (E) Crosssectioning
through embryos which induced partial secondary axis
by 10 pg noggin mRNA. (F) Embryos injected with 10 pg Xnr-1
mRNA and 10 pg Noggin mRNA. Complete (upper) or partial
(lower) secondary axes were induced. Xshh mRNAs were expressed
in both primary axis and secondary axis. (G) Crosssectioning
through the trunk of F. (H) Dorsal-expanded embryos
formed by injection with 10 pg Xnr-1 mRNA and 100 pg follistatin
mRNA. Xshh mRNAs were expressed in an ectopic manner on the
lateral side of the normal expression (asterisk). (I) Crosssectioning
through the trunk of H. Ectopic small notochord was
formed along the side of the primary neural tube. (J) Crosssectioning
through another dorsal-expanded embryo coinjected
Xnr-1 and follistatin mRNA. Large notochord was induced. Arrow
and arrowhead indicate primary and secondary axis, respectively.
Abbreviations: nc, notochord; fp, floor plate; nt, neural tissue. |
