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Fig. 1. Temporal and spatial expression of Xfoxi1a/b. Whole-mount in situ hybridization using albino embryos was performed with Xfoxi1a (A-C,E,F,I) or Xfoxi1b (J-L) probes. Double in situ hybridization was performed with (D) Xfoxi1a (turquoise; BCIP) and Sox2 (indigo; BM purple) probes, (G) Xfoxi1a (indigo; BM purple) and Sox2 (turquoise; BCIP) probes, and (H) Xfoxi1a (purple; magenta-phosphate) and Xsix1 (turquoise; BCIP) probes. (A) Animal view; (B) lateral view; (C,E,I,J,L) lateral view (anterior towards the left); (D,F,G,H,K) anterior view (dorsal towards the top). The embryo stage is shown in each panel. A, anterior; An, animal; D, dorsal; P, posterior; V, ventral; Vg, vegetal. (M) Amino acid sequence alignment of Xfoxi1a and Xfoxi1b. Identical and similar amino acid residues are marked with asterisks and double dots, respectively. Gaps are indicated by dashes. |
