Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
six1xenopus   

Too many results?Too few results?

Experiment details for six1

Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification.

Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification.

Gene Clone Species Stages Anatomy
six1.L laevis NF stage 13 anterior neural ridge
six1.L laevis NF stage 16 anterior neural fold , anterior neural ridge
six1.L laevis NF stage 19 anterior neural fold , anterior neural ridge
six1.L laevis NF stage 22 trigeminal ganglion , otic region
six1.L laevis NF stage 27 otic vesicle , trigeminal ganglion , olfactory region

Display additional annotations [+]
  Figure S4. Hairy2 overexpression does not induce major cell fate changes in the early embryo. (A) In neurula embryos, Hairy2-GR mRNA (500 pg) injection represses the expression of the MyT1, N-tubulin and NeuroD neuronal markers (a, 50% n = 22; b, 55% n = 18; c, 52% n = 27). No significant change is observed on the expression of neural plate (Sox3), mesodermal (MyoD), placodal (Six1, Six3, Pax8) and antero-posterior patterning markers (Otx2, HoxB9, XAG1). All unaffected: d, n = 15; e, n = 34; f, n = 14; g, n = 17; h, n = 24; i, n = 25; j, n = 16; k, n = 25; l, n = 20. Hairy2-GR mRNA (500pg) represses the epidermal marker Epi. K. (m, 64% n = 26 and expands transiently Sox2 at neurula stages (n, 66% increase n = 18) but have no effect on Sox2 at tailbud stages (o, 100% unaffected n = 19). (B) Hairy2-GR overexpression (500 pg) decreases N-tubulin (a, 75% n = 12; b, 53% n = 19; c, 55% n = 20; d, 50% n = 12) and does not affect Sox3 and Six1 expression in stage 13 to 26-27 embryos. (e-l, all unaffected; n = 20). Embryos are shown in dorso- anterior views with the injected side to the right (a-n) or in lateral view with inset showing the control side (o). β-galactosidase was used as a lineage tracer. (C) Q-PCR analysis of stage 14 NC induced animal caps derived from embryos injected with Hairy2 MO5mis (5 ng/blastomere), Hairy2 MOs (5 ng/blastomere) or Hairy2-GR mRNA (250 pg/blastomere). Note that both Hairy2 gain or loss of function reduces Slug (a) without significantly affecting Six1, Sox3 or N-tubulin expression. Ep. keratin expression which is strongly reduced in NC induced explants, appears also unaffected by Hairy2(c).