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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
six3
|
|
laevis
|
NF stage 19
|
pre-chordal neural plate
,
optic field
|
nrp1
|
|
laevis
|
NF stage 19
|
pre-chordal neural plate
,
chordal neural plate
,
trigeminal placode
,
neural plate
,
anterior neural fold
,
[+]
|
pax8
|
|
laevis
|
NF stage 19
|
lateral plate mesoderm
,
anterior dorsal lateral plate region
,
otic region
|
sox2
|
|
laevis
|
NF stage 19
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
sox2
|
|
laevis
|
NF stage 28
|
retina
,
forebrain
,
telencephalon
,
diencephalon
,
midbrain
,
[+]
|
sox3
|
|
laevis
|
NF stage 19
|
pre-chordal neural plate
,
chordal neural plate
,
trigeminal placode
,
neural plate
,
neural tube
|
sox3
|
|
laevis
|
NF stage 13
|
pre-chordal neural plate
,
chordal neural plate
,
trigeminal placode
,
neural plate
|
sox3
|
|
laevis
|
NF stage 16
|
pre-chordal neural plate
,
chordal neural plate
,
trigeminal placode
,
neural plate
,
neural tube
|
sox3
|
|
laevis
|
NF stage 22
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
posterior neural tube
,
[+]
|
sox3
|
|
laevis
|
NF stage 27
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
trigeminal ganglion
|
otx2
|
|
laevis
|
NF stage 19
|
pre-chordal neural plate
,
anterior neural fold
,
optic field
|
tubb2b
|
|
laevis
|
NF stage 19
|
trigeminal placode
,
Rohon-Beard neuron
,
neuron
|
tubb2b
|
|
laevis
|
NF stage 13
|
trigeminal placode
,
neuron
|
tubb2b
|
|
laevis
|
NF stage 16
|
trigeminal placode
,
Rohon-Beard neuron
,
neuron
|
tubb2b
|
|
laevis
|
NF stage 22
|
trigeminal ganglion
,
Rohon-Beard neuron
,
neuron
|
tubb2b
|
|
laevis
|
NF stage 27
|
trigeminal ganglion
,
Rohon-Beard neuron
,
neuron
|
neurod1
|
|
laevis
|
NF stage 19
|
trigeminal placode
,
neuron
|
myt1
|
|
laevis
|
NF stage 19
|
chordal neural plate
,
trigeminal placode
,
anterior neural fold
,
Rohon-Beard neuron
,
neuron
|
myod1
|
|
laevis
|
NF stage 19
|
presomitic mesoderm
|
ag1
|
|
xenopus
|
NF stage 19
|
cement gland primordium
|
krt12.4
|
|
laevis
|
NF stage 19
|
epidermis
,
non-neural ectoderm
|
hoxc9-like
|
|
laevis
|
NF stage 19
|
chordal neural plate
,
posterior
,
neural tube
|
|
|
Figure S4. Hairy2 overexpression does not induce major cell fate changes in the early embryo. (A) In neurula embryos, Hairy2-GR mRNA (500 pg) injection represses the expression of the MyT1, N-tubulin and NeuroD neuronal markers (a, 50% n = 22; b, 55% n = 18; c, 52% n = 27). No significant change is observed on the expression of neural plate (Sox3), mesodermal (MyoD), placodal (Six1, Six3, Pax8) and antero-posterior patterning markers (Otx2, HoxB9, XAG1). All unaffected: d, n = 15; e, n = 34; f, n = 14; g, n = 17; h, n = 24; i, n = 25; j, n = 16; k, n = 25; l, n = 20. Hairy2-GR mRNA (500pg) represses the epidermal marker Epi. K. (m, 64% n = 26 and expands transiently Sox2 at neurula stages (n, 66% increase n = 18) but have no effect on Sox2 at tailbud stages (o, 100% unaffected n = 19).
(B) Hairy2-GR overexpression (500 pg) decreases N-tubulin (a, 75% n = 12; b, 53% n = 19; c, 55% n = 20; d, 50% n = 12) and does not affect Sox3 and Six1 expression in stage 13 to 26-27 embryos. (e-l, all unaffected; n = 20). Embryos are shown in dorso- anterior views with the injected side to the right (a-n) or in lateral view with inset showing the control side (o). β-galactosidase was used as a lineage tracer.
(C) Q-PCR analysis of stage 14 NC induced animal caps derived from embryos injected with Hairy2 MO5mis (5 ng/blastomere), Hairy2 MOs (5 ng/blastomere) or Hairy2-GR mRNA (250 pg/blastomere). Note that both Hairy2 gain or loss of function reduces Slug (a) without significantly affecting Six1, Sox3 or N-tubulin expression. Ep. keratin expression which is strongly reduced in NC induced explants, appears also unaffected by Hairy2(c). |