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Fig. 4. RARalpha2 is required for expression of PPE genes. Embryos were injected unilaterally at the two- or four-cell stage. The injected side is on the right, as indicated by the magenta beta-galactosidase lineage tracer staining. (A,D,G,J) Control expression of Tbx1, Ripply3, Six1 and Eya1. (B,E,H,K) 10 ng RARalpha2 MO reduced expression of Tbx1 (14/15 embryos), Ripply3 (9/10), Six1 (6/11) and Eya1 (8/9). (C,F,I,L) 10 ng RARalpha2 MO + 0.5 ng RARalpha2 mRNA rescued expression of Tbx1 (5/11 embryos), Ripply3 (8/11), Six1 (11/14) and Eya1 (13/17). |
