| |
Fig. 5. Double whole-mount in situ hybridization reveals spatial relationship of PPE genes at stage 18. (A-C)Ripply3 (stained with BM Purple) is lateral to Tbx1, Six1 and Eya1 (stained with BCIP/Tetrazolium Blue), overlapping with Tbx1 and the posterior placodal (pp) area of Eya1. (D)Ripply3 (stained with Fast Red) is lateral to but does not overlap with Fgf8 (stained with BM-Purple). (E-G)Tbx1 (stained with BM Purple) is lateral to Fgf8, Six1 and Eya1 (all stained with Fast Red) in stage 18 embryos, overlapping significantly with Fgf8, and in the posterior placodal (pp) area of Eya1. |
