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Fig. 7. FGF8 establishes the PPE, but TBX1 maintains Fgf8 expression. All embryos were injected unilaterally at the two- or four- cell stage. The injected side is on the right. (A,C) Control expression of Tbx1 and Ripply3. (B,D) Embryos injected with 30 ng Fgf8 splice MO; Tbx1 (8/16 embryos) and Ripply3 (19/23) expression were knocked down or knocked out. (E,G,I,K) Embryos injected with 10 ng Tbx1 MO showed knockdown of Fgf8 (12/16 embryos), Ripply3 (17/21), Eya1 (21/26) and Six1 (25/36) expression. (F,H,J,L) 10 ng Tbx1 MO + 0.5 ng Tbx1 mRNA rescued Fgf8 (5/8), Ripply3 (6/8), Eya1 (6/8) and Six1 (8/11) expression. |
