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Fig. 3. δXTcf3 inhibits placode and anterior neural plate border markers. Embryos injected with mRNA encoding δXLef1 or δXTcf3 and the lineage tracer β-gal were examined by in situ hybridization (Stage 14) for cement gland and placodal markers with .denoting probe used. Inhibition of c-myc expression in δXLef1- and δXTcf3-injected embryos is not restricted to the neural crest forming regions; expression in the anterior/transverse neural fold is also inhibited (iv). The placodal component of the Sox3 expression domain is inhibited by δXLef1 (v, vi) and δXTcf3 (vii, viii). Six1 expression was largely unaffected by δXLef1 (ix, x), while FoxL1C expression was shifted more posterior in some (xii) but not all (xiv) δXLef1-injected embryos. δXTcf3 inhibited both Six1 (xi, xii) and FoxL1C (xv, xvi) expression. Both δXLef1 and δXTcf3 inhibited the cement gland marker, CG-1 (xviix), although δXTcf3 did so more potently. Arrow indicates injected side. Red stain is lineage tracer β-gal. |
