|
Gene |
Clone |
Species |
Stages |
Anatomy |
six1.L
|
|
laevis
|
NF stage 17
|
trigeminal placode
,
profundal placode
,
anterior placodal area
|
six1.L
|
|
laevis
|
NF stage 24
|
olfactory placode
,
otic placode
,
epibranchial placode
,
facial epibranchial placode
,
glossopharyngeal epibranchial placode
,
[+]
|
six1.L
|
|
laevis
|
NF stage 28
|
olfactory placode
,
otic vesicle
,
epibranchial placode
,
facial epibranchial placode
,
glossopharyngeal epibranchial placode
,
[+]
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pou3f2
|
|
laevis
|
NF stage 18
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
pou3f2
|
|
laevis
|
NF stage 24
|
brain
,
forebrain
,
midbrain
,
hindbrain
|
pou3f2
|
|
laevis
|
NF stage 28
|
otic vesicle
,
brain
,
forebrain
,
midbrain
,
hindbrain
,
[+]
|
tbx15
|
|
laevis
|
NF stage 18
|
neural crest
|
tbx15
|
|
laevis
|
NF stage 24
|
neural crest
,
somite
,
head somite
,
trunk somite
,
trunk neural crest
|
tbx15
|
|
laevis
|
NF stage 28
|
neural crest
,
somite
,
head somite
,
trunk somite
,
trunk neural crest
|
lhx5
|
|
laevis
|
NF stage 17
|
chordal neural plate
,
neural plate
|
lhx5
|
|
laevis
|
NF stage 24
|
forebrain
,
telencephalon
,
hindbrain
,
spinal cord
|
lhx5
|
|
laevis
|
NF stage 28
|
forebrain
,
telencephalon
,
hindbrain
,
spinal cord
|
emx1
|
|
laevis
|
NF stage 17
|
pre-chordal neural plate
,
chordal neural plate
,
neural plate
|
emx1
|
|
laevis
|
NF stage 28
|
forebrain
,
telencephalon
|
tbx6
|
|
laevis
|
NF stage 18
|
mesoderm
,
presomitic mesoderm
,
paraxial mesoderm
,
lateral plate mesoderm
|
tbx6
|
|
laevis
|
NF stage 24
|
presomitic mesoderm
,
somite
,
pharyngeal arch
,
hyoid arch
,
paraxial mesoderm
,
[+]
|
|
|
Figure 4—figure supplement 1.
Expression of targets not expressed in placodes in whole-mount Xenopus embryos.
Expression dynamics for each target are shown across a range of developmental stages: A1–F1 show expression in neural plate stage embryos, A2–F2 show early tail bud stage embryos and A3–F3 show late tail bud stage embryos. (A) Expression of PPE marker gene Six1 is shown as reference for placodal domains (for details see Pandur and Moody, 2000; Schlosser and Ahrens, 2004). (B) Emx1.2 is expressed broadly in the neural plate in neural plate stages (B1), and becomes restricted to the forebrain in late tail bud stages (B3; asterisk). (C) Lhx5 is expressed in the forebrain at all developmental stages (C1–C3; asterisk), and at early and late tail bud stages Lhx5 is also expressed in the hindbrain and spinal cord (C3; arrowhead). (D) Pou3f2b is expressed in the neural plate and developing neural tube (D1; asterisk) at neural plate stages. Expression in the brain and spinal cord is maintained during early and late tail bud stages (D2 and D3 ; arrowhead and asterisk, respectively). (E) Tbx15 is expressed in a restricted domain of the anterolateral neural folds in neural plate stages (E1; asterisk). At tail bud stages expression is prominent in somites (E2 and E3; arrowhead) and migrating neural crest cells of the hyoid and first branchial neural crest streams (Nc). Both of these expression domains are maintained into late tail bud stages (E3 and E4). E4 shows section at the level indicated in E3 (dotted line). Bar in E4: 100 μm. (F) Throughout all developmental stages (F1–F3) Tbx6 is expressed strongly in the posterior paraxial and lateral plate mesoderm (F1 and F2; asterisk) with weaker expression in the pharyngeal arches (F2; arrowhead). Subsequently, it’s expressed in somites, as indicated by a diamond in F2. Abbreviations: pA: anterior placodal region; pAD: anterior lateral line placode; pE: epibranchial placode; pM: middle lateral line placode; pO: olfactory placode; vOt: otic vesicle; pPrV: profundal/trigeminal placodes. |