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Fig. 5.
Loss and gain of Pa2G4 have different effects on the otocyst. (A) Two examples of pa2g4 morphants. In both, the dorsal-ventral diameter of the otocyst is smaller on the MO-injected side and gene expression levels are reduced. (B) In contrast, when Pa2G4 levels are increased by mRNA injection, the otocyst diameter is not significantly different from the control side of the same embryo, but gene expression levels also are reduced. (C) The dorsal-ventral diameters of pa2g4 morphants, measured in relative units by an eyepiece micrometer, were significantly smaller compared to the control side of the same embryo (n=13; p<0.00001, paired t-test), whereas they were not significantly different in embryos injected with pa2g4 mRNA (n=20; p>0.05).
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